The Shrine hosts more than 150 official commemorative services each year. They are held to remember the service of Australian men and women, particularly Victorians, in times of war and peacekeeping. The majority of these services take place:

• in the Sanctuary
• on the Second World War Memorial Forecourt
• at a Memorial Tree and its attendant plaque.

While each service is unique, each contains the following elements:

• laying of a wreath/wreaths
• recitation of Laurence Binyon’s 'Ode' from 'For the Fallen'
• the sounding of 'The Last Post' on a trumpet or bugle
• a period of silence
• 'Lest We Forget' (said by the MC, then repeated by all present)
• the sounding of the 'Rouse' on a trumpet or bugle
• playing of the Australian national anthem.

All ceremonial activities at the Shrine must be approved by the Trustees.